In recent years, moral personality has been one of the important topics in moral psychology and includes three areas of moral character, moral identity, and responsibility. Aim: The purpose of the research was to construct and validate the moral personality questionnaire based on Islamic sources and determine its psychometric properties. Method: The research method was Hsieh and Shannon's qualitative content analysis for analyze the data and survey method to check validity and reliability. The statistical population was students, from whom 212 people were selected as a sample using stratified random sampling. Results: Content validity was obtained for the findings and the items created by experts, each item with a minimum CVR of 0.99 and CVI index of 0.79 was selected. The validity of the structure was investigated by two methods of exploratory and confirmatory factor analysis with SPSS26 and Amos24 tools, and four factors of God-centered ethics, individual-centered ethics, other-oriented ethics, and environmental ethics factor were discovered and confirmed, and all the fit indices were sufficiently favorable. The validity of the questionnaire was obtained by the correlation score of each factor with the total score, Spearman-Brown coefficient, Gottman's two-half method and Cronbach's alpha. Cronbach's alpha was obtained for God-oriented ethics factor (0.83), person-oriented ethics (0.89), other-oriented ethics (0.89) and environmental ethics factor (0.83), which indicates a very good internal consistency of the questionnaire. Conclusion: By confirming the validity and reliability of the questionnaire, researchers can use the moral personality questionnaire based on Islamic sources in research and psychological research.